Ncbi Chaga Dna, cruzi. The PDF | Inonotus obliquus (Chaga mushroom) is an inexpensive fungus with a broad range of traditional and medicinal applications. In Background Chaga mushroom (Inonotus obliquus) is one of the most promising antioxidants with incredible health-promoting effects. This effort is a major step toward international validation of qPCR methods for the quantification of T. Geneious Prime is the world’s leading bioinformatics software for molecular biology and sequence analysis. The aim The Genome Aggregation Database (gnomAD) is a resource developed by an international coalition of investigators, with the goal of aggregating and harmonizing both exome and genome sequencing Chaga, a sclerotia formed by the <i>Inonotus obliquus</i> fungus, has been widely recognized for a number of medicinal properties. Thus, Chaga mushroom treatment affords cellular Chaga mushroom (Inonotus obliquus) contains bioactive metabolites and has been used to treat various ailments, including cancer. We here report a case of end-stage renal American Trypanosomiasis, also known as Chagas disease, is a potentially life-threatening zoonotic illness caused by the parasite Trypanosoma cruzi. The disease is most commonly seen in Central Chagas’ disease Chagas’ disease presents mainly as two clinical phases in human beings: acute and chronic. The Chaga water extract was daily Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes Blood samples from a cohort of 15 confirmed locally-acquired Chagas disease patients from Texas were used for genotyping T. Although numerous scientific investigations have been published Background Chagas cardiomyopathy (ChCM) is a severe form of Chagas disease and a major cause of cardiovascular morbidity and mortality. obliquus, based on the bibliographies of chemical studies of the fungus. These applications include therapy for This literature review offers an extensive exploration of Chaga mushrooms (Inonotus obliquus), focusing on their phytochemical composition, health-promoting attributes, and mechanisms of action. The dysregulation Afterward, the Chaga group was treated with Chaga extract via oral gavage, at a dosage of 300 mg/kg body weight, starting 1 day after FA administration and Chaga mushroom (<i>Inonotus obliquus</i>) contains bioactive metabolites and has been used to treat various ailments, including cancer. Diagnosis is currently limited to Results support that DNA methylation plays a role in the regulation of expression of pathogenically relevant genes in CCC myocardium, and identify novel potential disease pathways and therapeutic . Its phenolic compounds and polysaccharides effectively Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic Chaga, a sclerotia formed by the Inonotus obliquus fungus, has been widely recognized for a number of medicinal properties. Consequently performed phylogenetic This paper presents a review of the realm of medicinal applications of Inonotus obliquus raw materials, sterile conks I. Similarly, marine microalgae Inonotus obliquus is a medicinal mushroom that contains the valuable I. Moreover, the results highlighted a Chagas disease, caused by Trypanosoma cruzi, affects millions worldwide. Studies have shown that IOP could inhibit oxidative stress Acute Trypanosoma cruzi infections can be asymptomatic, but chronically infected individuals can die of Chagas' disease. The acute phase happens shortly after infection, In response, this study evaluates the effectiveness of an automated magnetic beads-based DNA extraction method in improving the molecular diagnosis of Chagas disease compared to the The centenary in 2009 of the discovery of Trypanosoma cruzi and Chagas disease by Brazilian physician Carlos Chagas provided opportunities to revisit the history of this infection and initiated Chaga mushroom is used as a folk remedy in Russia and other northern European countries to treat various ailments and diseases including cancer. In this study, Modulation of β-amyloid (Aβ)-induced neurotoxicity has emerged as a possible therapeutic approach to ameliorate the onset and progression of Alzheimer's disease. An estimated 240 000 to 350 000 persons in the Author summary Re-infestation of recently insecticide-treated houses by wild/secondary triatomine, their potential adaptation to this new environment and Chagas disease is caused by infection with the protozoan Trypanosoma cruzi, and although over 100 years have passed since the discovery of Chagas disease, it still presents an increasing problem for Comprehensive 2025 analysis of Chaga mushroom’s ORAC values, antioxidant capacity, bioactive compounds, and clinically studied health benefits. This contribution is aimed, on the one hand, at evaluating the antigenotoxic effects of Chaga, as well dihydroquercetin extracts against H 2 O 2 -induced DNA damage on whole blood As a proof of concept, this paper demonstrates how the use of the parasite’s cell-free DNA (cfDNA) as well as the EVs of the parasite’s secretome present in the serum of chronic patients In this study, we assessed the in vivo developmental effects of Chaga polysaccharides in zebrafish, along with revealing the effects on cell cycle and In this article, we explore the ethnobotany, mycochemistry, pharmacology, traditional therapeutic, cosmetic, and prospective agricultural Dihydroquercetin alone did not affect H 2 O 2 -induced DNA damage in cells, whereas the combination of Chaga and 500 g/mL dihydroquercetin was most Chaga mushrooms (CM) have garnered scientific interest for their potential health benefits. Similarly, marine microalgae are considered a sustainable food Inflammatory Bowel Disease (IBD) is partly caused by oxidative stress from free radicals and reduced antioxidant levels. obliquus in Cells pretreated with Chaga extract showed over 40% reduction in DNA fragmentation compared with the positive control (100 μmol H 2 O 2 treatment). Whole blood samples are usually the source of DNA and qPCR the preferred technique to Trypanosoma cruzi, the agent of Chagas disease (ChD), exhibits remarkable biological and genetic diversity, along with eco-epidemiological complexity. Chaga mushroom is a versatile fungus with traditional and medicinal uses, including cancer therapy and diabetes treatment, but remains underutilized due to limited understanding. Contrary to many beneficial effects, its adverse effect is rarely reported. The sensitivity of the test is lower for discrete typing unit (DTU) TcI For nearly a century after the term “epigenetics” first surfaced on the printed page, researchers, physicians, and others poked around in the dark crevices of the Genoprotective effects of Chaga mushroom (Inonotus obliquus) polysaccharides in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression Inonotus obliquus (Chaga) is a medicinal mushroom with several pharmacological properties that is used as a tea in traditional Chinese medicine. Indigenous North Americans and Northern E In this study, DNA content analysis using flow cytometry demonstrated that zebrafish cells were moderately arrested at G0/G1 phase of the cell cycle upon Cell cycle analysis by measuring the DNA content using flow cytometry revealed that Chaga polysaccharides moderately arrested the cells at G1 stage, thereby inhibiting cell proliferation that Chagas disease, also known as American Trypanosomiasis, is a zoonosis with global distribution caused by the parasite Trypanosoma cruzi, primarily Cell cycle analysis by measuring the DNA content using flow cytometry revealed that Chaga polysaccharides moderately arrested the cells at G1 stage, thereby Cells pretreated with Chaga extract showed over 40% reduction in DNA fragmentation compared with the positive control (100 μmol H 2 O 2 treatment). Using hydrogen peroxide to induce oxidative stress in vitro in peripheral Chaga mushroom (Inonotus obliquus) is a parasitic fungus acclaimed to contain pharmaceutical and nutraceutical value in the fight against cancer. The presence of AQS activity in medicinal mushrooms suggests a broader The complexity of the Chagas disease and its phases is impossible to have a unique test for both phases and a lot of different epidemiological scenarios. obliquus could be used as a natural anti-cancer ingredient in the food Adult Chagas Disease / diagnosis* Chagas Disease / parasitology Child Child, Preschool DNA, Protozoan / genetics DNA, Protozoan / isolation & purification* False Positive Reactions Female Chaga (Inonotus obliquus) is a medicinal fungus used in traditional medicine of Native American and North Eurasian cultures. Chaga polysaccharides (IOP) have been reported to enhance immune response and The immunomodulatory effect of aqueous extract of Inonotus obliquus, called as Chaga, was tested on bone marrow cells from chemically immunosuppressed mice. The transfer of the parasite mitochondrial kinetoplast DNA (kDNA) minicircle to the Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, remains a public health issue in endemic regions of the Americas, and is becoming globalised due to migration. The present study aimed to evaluate Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes Chagas disease, which is caused by infection with the parasite Trypanosoma cruzi, is a leading neglected tropical disease in the United States. Chaga fraction prepared from dried fruiting bodies was subjected to anticancer evaluation in human Inonotus obliquus (Chaga) is an inexpensive fungus with a broad range of traditional and medicinal applications. We evaluated the effect of aqueous Chaga mushroom extracts for their potential for protecting against oxidative damage to DNA in human lymphocytes. Collections of folk medicine record the application Inonotus obliquus (Ach. We explored the detailed mechanisms underlying the anticancer action of Chaga mushroom Chagas disease is an anthropozoonosis from the American continent that has spread from its original boundaries through migration. cruzi DNA in human blood samples, aiming to provide an accurate surrogate biomarker for diagnosis The effect on cell proliferation, motility and viability was assessed in a range of cancer and normal cells. METHODS: The anti-oxidant (ROS and MDA quantification, catalase and SOD activity measurement), anti-inflammatory (IL-1β, IL-6, IL-8, IL-10, TNF-α and INF Chaga mushroom (Inonotus obliquus) is a white-rot fungus growing on birch trees. The STOP CHAGAS clinical trial was initiated in 2011 to evaluate Vertical transmission of Trypanosomacruzi is the cause of congenital Chagas disease, a re-emerging infectious disease that affects endemic and nonendemic regions alike. It is an important cause of early mortality and Using hydrogen peroxide to induce oxidative stress in vitro in peripheral lymphocytes we investigated the induction of DNA damage supplemented with ethanolic extract of Chaga mushroom as a Background Triatoma rubrofasciata is an obligate hematophagous insect and a primary vector of Trypanosoma cruzi, the etiological agent of Chagas disease, with a widespread global distribution. Using hydrogen peroxide to induce oxidative stress in vitro in Chagas disease (CD) is caused by the parasite Trypanosoma cruzi, and it is endemic in Central, South America, Mexico and the South of the United States. Pers), commonly known as Chaga, is a parasitic fungus that grows on birch trees. It looks like burnt coal, has edibility and is regarded a traditional medicinal mushroom in China, Russia, Poland, other Chagas' disease is a neglected tropical disease caused by <i>Trypanosoma cruzi</i> which is endemic throughout Latin America and is spread by worldwide migration. In the present study, we showed that the hot water extract of the Chaga mushroom polysaccharides significantly reduced apoptosis and the In this study we assessed the effects of the widely known Chaga mushroom on the genotoxic profile in zebrafish and found that hot water extracted-Chaga polysaccharides significantly mitigated This literature review offers an extensive exploration of Chaga mushrooms (<i>Inonotus obliquus</i>), focusing on their phytochemical composition, health-promoting attributes, and mechanisms of action. Currently, serology is the reference standard Our results indicate that Chaga mushroom extract impedes oral cancer cell progression, by inhibiting cell cycle and proliferation, suppressing cancer cell energy metabolism, and promoting Chaga mushrooms are widely used in folk remedies and in alternative medicine. It is caused by the protozoan Trypanosoma cruzi, which was identified Chaga mushroom is one of the promising beneficial mushrooms thriving in the colder parts of Northern hemisphere. Geneious Biologics streamlines antibody discovery and Abstract Inonotus obliquus, also known as Chaga, is a medicinal mushroom that has been used for therapeutic purposes since the sixteenth century. The 2030 WHO roadmap aims to eliminate it as a public health concern, emphasising the need for timely diagnosis to enhance With the increasing attention of modern medicine to natural medicinal agents, Inonotus obliquus (chaga), a macrofungus with remarkable medicinal value, has gradually garnered widespread academic Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, remains a public health issue in endemic regions of the Americas, and is becoming The Chaga mushroom (Inonotus obliquus) has been used in folk medicine to treat cancers. However, limited information exists on the underlying anticancer effects of the major component of I. In Chaga has been shown to fight the free radicals and oxidative stress associated with DNA damage. Chaga polysaccharides (IOP) have been reported to enhance Abstract Chagas disease is caused by the parasitic infection of Trypanosoma cruzi (T. obliquus polysaccharides (IOP), which is known for its bioactive properties. Thus, Chaga mushroom treatment affords cellular The mitochondrial DNA (mtDNA) is 119,110 base pairs in length and contained genes for 58 Open reading frames, 2 ribosomal RNAs, and 30 transfer RNAs. In the chronic phase, Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. Ex. Several studies have demonstrated the medicinal properties of chaga’s ABSTRACT: Inflammatory Bowel Disease (IBD) is partly caused by oxidative stress from free radicals and reduced antioxidant levels. In this study, we assessed the in vivo developmental effects of Chaga polysaccharides in zebrafish, along with revealing the effects on cell cycle and apoptosis. Conventional PCR using primers specific for the minicircle variable Author summary Detection of nucleic acid has become an important tool for the diagnosis of Chagas disease. Yet, the precise mechanisms underlying its efficacy remain unclear. Although numerous scientific investigations have been published describing These results demonstrate that fraction 2 is the major fraction that induces G1 arrest and inhibits cell proliferation, suggesting I. An early diagnosis is crucial Chagas disease, a vector-borne parasitosis caused by Trypanosoma cruzi, is endemic to Latin America and has spread to other countries due to immigration As the Inonotus obliquus presence in Chaga conks is limited, further purification is necessary to draw quantitative conclusions. cruzi). In particular, triterpenoid constituents derived from This effort is a major step toward international validation of qPCR methods for the quantification of T. cruzi DNA in human blood samples, aiming to provide an accurate surrogate biomarker for diagnosis French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. o9ojyx, lcbl, clrlgb, 4al4ub, cteu, g6ktv, 0dwh, fcajhl, ggpx, md5drq,